Feline Panleucopenia/Herpesvirus/Calicivirus Antibody Combo Test

The Felivet FPV-FHV-FCV Ab Combo Test is a lateral flow immunochromatographic assay for the semi-quantitative detection of  FPV-FHV-FCV Antibody (FPV-FHV-FCV Ab) in cat's serum, or plasma.

Assay Time:  10 minutes

Specimen: serum or plasma

Principle
The Felivet FPV-FHV-FCV Ab Combo Test is based on sandwich lateral flow immunochromatographic assay. The test card has a testing window for the observation of assay running and result reading. The testing window has an invisible T (test) zone and a C (control) zone before running the assay. When the treated sample was applied into the sample hole on the device, the liquid will laterally flow through the surface of the test strip and react with the pre-coated FPV-FHV-FCV recombinant antigens. If there are   FPV-FHV-FCV antibodies in the specimen, a visible T line will appear. The C line should always appear after a sample is applied, which indicates a valid result. By this means, the device can indicate the presence of  FPV-FHV-FCV antibodies in the specimen.

Test Procedure-Allow all materials, including specimen and test device, recover to 15-25ºC before running the assay.
-Take out the test device from the foil pouch and place it horizontally.
-Drop 1 drops (approx. 40μL)of the serum or plasma specimen into the sample hole "S" of the test device then drop two drops(approx. 80μL) of buffer.
-Interpret the result in 10 minutes. Result after 15 minutes is considered as invalid.
-The sequence of the T line is shown in the figure.

Interpretation Of Result
The presence of "C" line indicates a valid result. Read the result by comparing the color intensity of the "T" line with the standard color reference scale (from "G1" to "G6").
The titers of FPV-FHV-FCV antibodies are corresponding with the color intensity grades.

-Positive (+): The presence of both "C" line and zone "T" line. T line at G3 is considered as standard positive. T line < G3, is weak positive and indicates low FPV-FHV-FCV antibody level. T line around G4-G5, is strong positive. T line > G6, is very strong positive, indicates a contact with virus recently or in infection.
-Negative (-): Clear C line appears. No T line, or T line is weaker than G1. It indicates very low FPV-FHV-FCV antibody titer. Failed immunization may happen.   
-Invalid: No colored line appears in C zone.