Short Introduction
The Felivet FCV-FHV-FPV Ag Combo Test is a lateral flow immunochromatographic assay for the differential diagnosis of Feline Calicivirus virus antigen (FCV Ag), Feline Herpesvirus Type-1 antigen (FHV Ag) and Feline Panleukopenia virus antigen (FPV Ag) in cat's specimen.
Assay Time: 5-10 minutes
Specimen: FCV-FHV Ag--- secretions from cat's eyes, nasal cavities, and anus.
FPV Ag--- feces or vomit
Principle
The Felivet FCV-FHV-FPV Ag Combo Test is based on sandwich lateral flow immunochromatographic assay. The test card has two testing windows for the observation of assay running and result reading. The testing window has an invisible T (test) zone and a C (control) zone before running the assay. When the treated sample was applied into the sample hole on the device, the liquid will laterally flow through the surface of the test strip and react with the pre-coated monoclonal antibodies. If there is FCV, FHV or FPV antigen in the specimen, a visible T line will appears. In this way, the device can accurately indicate the presence of Calicivirus virus antigen, Herpesvirus Type-1 antigen, Panleukopenia virus antigen in the specimen.
Test Procedure-Allow all materials, including specimen and test device, recover to 15-25ºC before running the assay.
FCV-FHV Ag test procedure-Collect cat's ocular, nasal or anus secretions with the swab stick and make the swab wet sufficiently.
-Insert the swab into the provided assay buffer tube. Agitates it to get efficient sample extraction.
-Take out the test device from the foil pouch and place it horizontally.
-Suck the treated sample extraction from the assay buffer tube and place 3 drops into the sample hole "S" of the test device.
-Interpret the result in 5-10 minutes. Result after 10 minutes is considered as invalid.
https://image.made-in-china.com/226f3j00ScsozIbClfqn/Fcv-Fhv-Fpv-AG-Triple-Test-Feline-Calicivirus-Herpesvirus-Type-1-Panleukopenia-Antigen-Rapid-Test.webp
FPV Ag test procedure
-Collect cat's fresh feces or vomit with the swab from cat's anus or from the ground.
-Insert the swab into the provided assay buffer tube. Agitates it to get efficient sample extraction.
-Take out the test device from the foil pouch and place it horizontally.
-Suck the treated sample extraction from the assay buffer tube and place 4 drops into the sample hole "S" of the test device.
Note: If the liquid will not flow through the surface of the test strip in 30 seconds, please add another drop of the treated sample extraction.-Interpret the result in 5-10 minutes. Result after 10 minutes is considered as invalid.
Interpretation Of Result-Positive (+): The presence of both "C" line and zone "T" line, no matter T line is clear or vague.
-Negative (-): Only clear C line appear. No T line.
-Invalid: No colored line appears in C zone. No matter if T line appears.