Feline Herpesvirus/Calicivirus/Mycoplasma Pneumonia Antigen Combo Test

Short Introduction
The upper respiratory tract of cats mainly includes the nose and throat. When the upper respiratory tract is infected, cats will have symptoms such as cough, runny nose, frequent sneezing and fever. Some cats will have increased eye discharge, mostly purulent discharge.

The Felivet FHV-FCV-MP Ag Combo Test is a lateral flow immunochromatographic assay for the differential diagnosis of Feline Herpesvirus Type-1 antigen (FHV Ag), Feline Calicivirus virus antigen (FCV Ag) and Feline Mycoplasma Pneumoniae antigen (MP Ag) in secretions from cat's eyes and nasal cavities.

Principle
The Felivet FHV-FCV-MP Ag Combo Test is based on sandwich lateral flow immunochromatographic assay. The test card has two testing windows for the observation of assay running and result reading. The testing window has an invisible T (test) zone and a C (control) zone before running the assay. When the treated sample was applied into the sample hole on the device, the liquid will laterally flow through the surface of the test strip and react with the pre-coated monoclonal antibodies. If there is FHV, FCV or MP antigens in the specimen, a visible T line will appears. In this way, the device can accurately indicate the presence of FHV, FCV and MP antigen in the specimen.

Test Procedure-Allow all materials, including specimen and test device, recover to 15-25ºC before running the assay.
-Collect cat's ocular or nasal secretions with the swab stick and make the swab wet sufficiently.
-Insert the swab into the provided assay buffer tube. Agitates it to get efficient sample extraction.
-Take out the test device from the foil pouch and place it horizontally.
-Suck the treated sample extraction from the assay buffer tube and place 3 drops into the sample hole "S" of the test device.
-Interpret the result in 5-10 minutes. Result after 15 minutes is considered as invalid.

Interpretation Of Result-Positive (+): The presence of both "C" line and zone "T" line, no matter T line is clear or vague.
-Negative (-): Only clear C line appear. No T line.
-Invalid: No colored line appears in C zone. No matter if T line appears.