Short Introduction
Canine coronavirus can infect all breeds and dogs of all ages (especially puppies), causing gastroenteritis symptoms of varying severity, frequent vomiting, diarrhea, depression, anorexia in clinical practice, 14 to 21 days after the clinical symptoms disappeared, can still relapse, is a major infectious disease endangering the dog industry.
The route of infection is the digestive tract, which is mainly caused by contact with faeces and contaminants from sick dogs.
The Canivet CCV Ag Test is a lateral flow immunochromatographic assay for the qualitative detection of canine parvo virus antigen (CCV Ag) in dog's feces or vomit specimen.
PrincipleThe Canivet CCV Ag Test is based on sandwich lateral flow immunochromatographic assay. The test device has a testing window for the observation of assay running and result reading. The testing window has an invisible T (test) zone and a C (control) zone before running the assay. When the treated sample was applied into the sample hole on the device, the liquid will laterally flow through the surface of the test strip and react with the pre-coated monoclonal antibodies. If there is CCV antigen in the specimen, a visible T line will appear. The C line should always appear after a sample is applied, which indicates a valid result. By this means, the device can accurately indicate the presence of CCV antigen in the specimen.
Test Procedure-Collect dog's fresh feces or vomit with the cotton swab stick from dog's anus or from the ground.
-Insert the swab into the provided assay buffer tube. Agitates it to get efficient sample extraction.
-Take out the test card from the foil pouch and place it horizontally.
-Suck the treated sample extraction from the assay buffer tube and place 3 drops into the sample hole "S" of the test device.
-Interpret the result in 5-10 minutes. Result after 10 minutes is considered as invalid.
Interpretation Of Result
- Positive (+): The presence of both "C" line and zone "T" line, no matter T line is clear or vague.
- Negative (-): Only clear C line appear. No T line.
- Invalid: No colored line appears in C zone. No matter if T line appears.