Ehrlichia-BAbesia gibsoni-Anaplasma-Leptospira Antibody Combo Test

Short Introduction

The Camanava kit EHR-BAB-ANA-LEPTO Ab Combo Test is a lateral flow immunochromatographic assay for the qualitative detection of Ehrlichia canis antibodies (E.canis Ab), Babesia gibsoni antibodies, Anaplasma spp. Antibodies and Leptospira spp. antibodies (LEPTO Ab) in dog's serum, plasma and whole blood specimen.

Principle
The Camanava kit EHR-BAB-ANA-LEPTO Ab Combo Test is based on sandwich method lateral flow immunochromatographic assay. The testing window has an invisible T (test) zone and a C (control) zone before running the assay. When the treated sample was applied into each sample hole on the device, the liquid will laterally flow through the surface of the test strip and react with the pre-coated Ehrlichia, Babesia, Anaplasma and Leptospira recombinant antigens respectively. If there are Ehrlichia, Babesia, Anaplasma and Leptospira antibodies in the specimen, a visible T line will appear. By this means, the device can accurately indicate the presence of Ehrlichia, Babesia, Anaplasma and Leptospira antibodies in the specimen.

Test Procedure-Allow all materials, including specimen and test device, recover to 15-25ºC before running the assay.-Take out the test card from the foil pouch and place it horizontally.
-Use the capillary dropper to place 1 drop (approx.10μL) of the prepared specimen into each sample hole "S" of the test card. Then drop 3 drops (approx. 90μL) of the assay buffer into each sample hole. Start the timer.  
-Interpret the result in 5-10 minutes. Result after 10 minutes is considered as invalid.

Interpretation Of Result
- Positive (+): The presence of both "C" line and zone "T" line, no matter T line is clear or vague.
- Negative (-): Only clear C line appear. No T line.
- Invalid: No colored line appears in C zone. No matter if T line appears.