Feline Calicivirus-Herpesvirus Type-1-Coronavirus-Panleukopenia Virus Antigen Combo Test

Feline Calicivirus Herpesvirus Type-1 Antigen Combo Rapid Test is a lateral flow immunochromatographic assay for the qualitative detection of Feline Calicivirus antigen (FCV Ag) and Feline Herpesvirus Type-1 antigen (FHV Ag) in secretions from cat's eyes, nasal cavities, and anus or in serum, plasma specimen.

Principle

Feline Calicivirus Herpesvirus Type-1 Antigen Combo Rapid Test is based on sandwich lateral flow immunochromatography. The test device has a test window for viewing assay runs and reading results. Before the assay, the test window has an invisible T (test) area and C (control) area. When the processed sample is applied to the sample well on the device, the liquid will flow laterally across the surface of the test strip and react with the pre-coated monoclonal antibody. Visible T-lines appear if FCV antigen or FHV antigen is present in the specimen. Line C should always appear after applying an example, which indicates a valid result. In this way, the device can accurately indicate the presence of FCV antigen or FHV antigen in the specimen

Reagents and materials

10 test pouches, with cards and disposable droppers
1 package insert
1 Standard color card
A bottle of buffer

Test procedure

- Collect cat's eye, nose or anal secretions with a cotton swab and moisten the cotton swab well.
- Insert the swab into the provided assay buffer tube. Stir it for efficient sample extraction.
- Take the test unit out of the foil bag and place it horizontally.
- Aspirate the processed sample from the assay buffer tube and place 3 drops into the sample well "S" of the test device.
Interpret the results in 5-10 minutes. Results after 10 minutes are considered invalid.

INTERPRETATION OF RESULTS
- Positive (+): The presence of both “C” line and zone “T” line, no matter T line is clear or vague.
- Negative (-): Only clear C line appear. No T line.
- Invalid: No colored line appears in C zone. No matter if T line appears.

 

 

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