Feline Pancreatic Lipase Rapid Test

fPL Test Feline Pancreatic Lipase Rapid Test kit is a lateral flow immunochromatographic assay for the qualitative detection of the feline pancreatic lipase (fPL) in cat’s serum, plasma or whole blood specimen, as an auxiliary examination of pancreatitis.

Cutoff: 4μg/L

Assay Time: 5-10 minutes

PRINCIPLE

fPL Test Feline Pancreatic Lipase Rapid Test kit is based on sandwich method lateral flow immunochromatographic assay. The test card has a testing window for the observation of assay running and result reading. The testing window has an invisible T (test) zone and a C (control) zone before running the assay. When the treated sample was applied into the sample hole on the device, the liquid will laterally flow through the surface of the test strip and react with the pre-coated fPL monoclonal antibodies. If the concentration of fPL is exceeding the cutoff , a visible T line will appear. The C line should always appear after a sample is applied, which indicates a valid result. By this means, the device can accurately indicate the presence of fPL antigen in the specimen.

REAGENTS AND MATERIALS

- 10 test pouches, with test cards and desiccants
- 10 capillary droppers
- 1 bottle of assay buffer
- 1 package insert

TEST PROCEDURE FOR fPL Test Feline Pancreatic Lipase Rapid Test kit


Allow all materials, including specimen and test device, recover to 15-25℃ before running the assay.

- Take out the test card from the foil pouch and place it horizontally.
- Use the capillary dropper to suck specimen and place 1 drop (approx. 10μL) of the prepared specimen into sample hole “S” of the test card. Then drop 3 drops (approx. 90μL) of the assay buffer into the sample hole. Start the timer.
- Interpret the result in 5-10 minutes. Result after 10 minutes is considered as invalid.