Feline Immunodeficiency Virus Antibody-Feline Leukemia Virus Antigen-Feline Coronavirus Antibody Combo Test

Short Introduction
 The Felivet GIA-TRF Ag Combo Test is a test cassette to diagnose the presence of antigens against Giardia (GIA) and Tritrichomonas foetus (TRF) in cat's feces specimen.

Principle
The Felivet FIV Ab-FELV Ag-FCOV Ab Combo Test is based on sandwich method lateral flow immunochromatographic assay. The test card has a testing window for the observation of assay running and result reading. The testing window has an invisible T (test) zone and a C (control) zone before running the assay. When the treated sample was applied into each sample hole on the device, the liquid will laterally flow through the surface of each test strip and react with the pre-coated FIV recombinant antigens, FeLV antibodies and FCoV recombinant antigens respectively. If there are FIV antibodies, FeLV antigens and FCoV antibodies in the specimen, a visible T line will appear in the corresponding window. The C line should always appear after a sample is applied, which indicates a valid result. By this means, the device can accurately indicate the presence of FIV antibodies, FeLV antigens and FCoV antibodies in the specimen.

Test ProcedureFIV Ab-FeLV Ag-Allow all materials, including specimen and test device, recover to 15-25ºC before running the assay.
-Take out the test card from the foil pouch and place it horizontally.
-Collect 50μL of the prepared specimen into a vial of assay buffer and mix well. Then drop 3 drops (approx. 120μL) of the diluted sample into each sample hole "S" of the test card, respectfully corresponding to the windows FIV, FeLV. Start the timer.
-Interpret the result in 5-10 minutes. Result after 10 minutes is considered as invalid.

FCoV Ab-Allow all materials, including specimen and test device, recover to 15-25ºC before running the assay.
-Take out the test device from the foil pouch and place it horizontally.
-Using the capillary dropper to place 1 drop of the prepared specimen into the sample hole "S" of the test device. Then drop 3 drops of the assay buffer into the sample hole immediately.
-Interpret the result in 5-10 minutes. Result after 10 minutes is considered as invalid.

Interpretation Of Result-Positive (+): The presence of both "C" line and zone "T" line, no matter T line is clear or vague.
-Negative (-): Only clear C line appear. No T line.
-Invalid: No colored line appears in C zone. No matter if T line appears.